Molecular Cell Biology
Eighth Edition   ©2016

Molecular Cell Biology

Harvey Lodish (Massachusetts Institute of Technology) , Arnold Berk (University of California, Los Angeles) , Chris A. Kaiser (Massachusetts Institute of Technology) , Monty Krieger (Massachusetts Institute of Technology) , Anthony Bretscher (Cornell University) , Hidde Ploegh (Massachusetts Institute of Technology) , Angelika Amon (Massachusetts Institute of Technology) , Kelsey C. Martin (University of California, Los Angeles)

  • ISBN-10: 1-4641-8339-2; ISBN-13: 978-1-4641-8339-3; Format: Cloth Text

New Discoveries and Methodologies in 8e

1. Molecules, Cells, and Model Organisms

• Model organisms Chlamydomonas reinhardtii (for study of flagella, chloroplast formation, photosynthesis, and phototaxis) and Plasmodium falciparum (novel organelles and a complex life cycle) 

3. Protein Structure and Function
• Intrinsically disordered proteins
• Chaperone-guided folding and updated chaperone structures
• Unfolded proteins and the amyloid state and disease
• Hydrogen/deuterium exchange mass spectrometry (HXMS)
• Phosphoproteomics 

4. Culturing and Visualizing Cells
• Two-photon excitation microscopy 
• Light sheet microscopy 
• Super resolution microscopy
• 3D culture matricies and 3D printing

5. Fundamental Molecular Genetic Mechanisms
• Ribosome structural comparison across domains shows conserved core 

6. Molecular Genetic Techniques
• CRISPR/Cas9 system in bacteria and its application in genomic editing 

7. Biomembrane Structure

8. Genes, Genomics, and Chromosomes
• Chromosome conformation capture techniques reveal topological domains in chromosome territories within the nucleus 

9. Transcriptional Control of Gene Expression
• DNase I hypersensitivity mapping reveals cell developmental history
• Long non-coding RNAs involved in X-inactivation in mammals 
• ENCODE databases 

10. Post-transcriptional Gene Control
• Improved discussion of mRNA degradation pathways and RNA surveillance in the cytoplasm
• Nuclear bodies: P bodies, Cajal bodies, histone locus bodies, speckles, paraspeckles, and PML-nuclear bodies

11. Transmembrane Transport of Ions and Small Molecules
• GLUT1 molecular model and transport cycle 

13. Moving Proteins into Membranes and Organelles
• Expanded discussion of the pathway for import of PTS1-bearing proteins into the peroxisomal matrix

14. Vesicular Traffic, Secretion, and Endocytosis
• Expanded discussion of Rab proteins and their role in vesicle fusion with target membranes

15. Signal Transduction and G Protein–Coupled Receptors
• Human G protein-coupled receptors of pharmaceutical importance

16. Signaling Pathways That Control Gene Expression
• Wnt concentration gradients in planaria development and regeneration
• Inflammatory hormones in adipose cell function and obesity
• Regulation of insulin and glucagon function in control of blood glucose

17. Cell Organization and Movement I: Microfilaments
• Use of troponins as an indicator of the severity of a heart attack

18. Cell Organization and Movement II: Microtubules and Intermediate Filaments 
• Neurofilaments and keratins involved in skin integrity, epidermolysus bullosa simplex
• New structures and understanding of function of dynein and dynactin

19. The Eukaryotic Cell Cycle

• The Hippo pathway 
• Spindle checkpoint assembly and nondisjunction and aneuploidy in mice, and nondisjunction increases with maternal age

Part IV. Cell Growth and Differentiation
20. Integrating Cells Into Tissues

• Expanded discussion of the functions of the extracellular matrix and the role of cells in assembling it
• Mechanotransduction
• Structure of cadherins and their cis and trans interactions
• Cadherins as receptors for Class C rhinoviruses and asthma
• Improved discussion of microfibrils in elastic tissue and in LTBP-mediated TGF-B signaling
• Tunneling nanotubes
• Functions of WAKs in plants as pectin receptors

21. Stem Cells, Cell Asymmetry, and Cell Death
• Pluripotency of mouse ES cells and the potential of differentiated cells derived from iPS and ES cells in treating various diseases
• Pluripotent ES cells in planaria
• Cells in intestinal crypts can dedifferentiate to replenish intestinal stem cells
• Cdc42 and feedback loops that control cell polarity

22. Cells of the Nervous System
• Prokaryotic voltage-gated Na+ channel structure, allowing comparison with voltage-gated K+ channels
• Optogenetics techniques for linking neural circuits with behavior
• Mechanisms of synaptic plasticity that govern learning and memory

23. Immunology

• Inflammasomes and non-TLR nucleic sensors
• Expanded discussion of somatic hypermutation
• Improved discussion of the MHC molecule classes, MHC-peptide complexes and their interactions with T-cells
• Lineage commitment of T cells
• Tumor immunology

24. Cancer

• The characteristics of cancer cells and how they differ from normal cells
• How carcinogens lead to mutations and how mutations accumulate to cancer