Seventh Edition   ©2017

Lehninger Principles of Biochemistry

David L. Nelson (University of Wisconsin-Madison) , Michael M. Cox (University of Wisconsin-Madison)

  • ISBN-10: 1-4641-2611-9; ISBN-13: 978-1-4641-2611-6; Format: Cloth Text, 1312 pages

NEW Leading-Edge Science Topics, including
    ► Synthetic cells and disease genomics (Chapter 1)
    ► Intrinsically disordered protein segments (Chapter 4)
    ► Pre-steady state enzyme kinetics (Chapter 6)
    ► Gene annotation (Chapter 9)
    ► Gene editing via CRISPR (Chapter 9)
    ► Membrane trafficking and dynamics (Chapter 11)
    ► Importance of intrinsically disordered proteins in signaling (Chapter 12)
    ► New roles for NADH (Chapter 13)
    ► Specialized pro-resolving mediators (Chapter 21)
    ► Peptide hormones: incretins and blood glucose, irisin and exercise (Chapter 23)
    ► Chromosomal territories (Chapter 24)
    ► New details of eukaryotic DNA replication (Chapter 25)
    ► Cap snatching; spliceosome structure (Chapter 26)
    ► Ribosome rescue; RNA editing update (Chapter 27)
    ► New roles for non-coding RNAs (Chapters 26 and 28)
    ► The RNA recognition motif (Chapter 28)
NEW Tools and Technology, including
   ► Next-generation DNA sequencing now includes ion-torrent and single molecule real time (SMRT) sequencing platforms, and the discussion has moved to follow classical Sanger sequencing (Chapter 8). 
   ► Gene editing via CRISPR is one of many updates to the discussion of genomics (Chapter 9).
   ► LIPID MAPS database and system of classifying lipids is included in the discussion of lipidomics (Chapter 10).
   ► Cryo-electron microscopy is described in a new box (Chapter 19).
   ► Ribosome profiling to determine which genes are being translated at any given moment, and many related technologies, are included to illustrate the versatility and power of deep DNA sequencing (Chapter 27)
   ► Online data resources such as NCBI, PDB, SCOP2, KEGG, and BLAST, mentioned throughout the text, are listed in the back endpapers for easy reference.
NEW Chapter 20, Carbohydrate Biosynthesis in Plants and Bacteria
All coverage of plant metabolism is now consolidated into a single chapter, separate from oxidative phosphorylation, Chapter 19. Chapter 20 includes light-driven ATP synthesis, carbon fixation, photorespiration, the glyoxylate cycle, cellulose synthesis, and regulatory mechanisms that ensure integration of all of these activities throughout the plant.
NEW Medical Insights and Applications <Medical icon>
   ► NEW Guillain-Barré syndrome and gangliosides (Chapter 10)
   ► NEW Golden Rice Project to prevent diseases of vitamin A deficiency (Chapter 10)
   ► NEW Insight into cystic fibrosis and its treatment (Chapter 11)
   ► NEW Newborn screening for acyl-carnitine to diagnose mitochondrial disease (Chapter 17)
   ► NEW Mitochondrial diseases, mitochondrial donation, and “three-parent babies” (Chapter 19) 
   ► NEW Xenobiotics as endocrine disruptors (Chapter 23)
NEW Special Theme: Metabolic Integration, Obesity and Diabetes
The focus on diabetes provides an integrating theme throughout the chapters on metabolism and its control. In this edition:
   ► NEW Na+-glucose transporter and the use of gliflozins in the treatment of type 2 diabetes (Chapter 11)
   ► NEW Breakdown of amino acids: methylglyoxal as a contributor to type 2 diabetes (Chapter 18)
   ► NEW  AMP-activated protein kinase in hypothalamus integrates hormonal inputs from gut, muscle, and adipose tissues (Chapter 23)
   ► NEW Brown and beige adipose tissues are thermogenic (Chapter 23)
   ► NEW Exercise stimulates irisin release and weight loss (Chapter 23)
   ► NEW Short-term eating behavior is influenced by ghrelin, PYY3–36, and cannabinoids (Chapter 23)
   ► NEW Microbial symbionts in the gut influence energy metabolism and adipogenesis (Chapter 23)
NEW Sapling Plus for Lehninger
This comprehensive and robust online platform combines the innovative high quality teaching and learning features with Sapling Learning’s acclaimed online homework for biochemistry:
Proven effective at raising students’ comprehension and problem-solving skills and recently updated with hundreds of additional questions, Sapling Learning‘s innovative online homework for biochemistry offers:
Immediate, Individualized Feedback
Each student gets the guidance they need when they need it.
Efficient Course Management
Automatic grading, tracking, and analytics helps instructors save time and tailor assignments to student needs.
Industry-Leading Peer-to-Peer Support
Each instructor is paired with Tech TA—a fully trained PhD or Masters level colleague ready to help with everything from quizzes and assignments to syllabus planning and tech support.
Students are provided with media designed to enhance their understanding of biochemical principles and improve their problem-solving ability, including:
A fully interactive e-Book
The e-Book contains the full contents of the text and links to all student media integrated at point of use.
NEW Interactive Metabolic Map
With this interactive tool, students can navigate and zoom between overview and detailed views of the most commonly taught metabolic pathways—glycolysis, gluconeogenesis, the citric acid cycle, oxidative phosphorylation, the penthose phosphate pathway, fatty acid synthesis, and β-oxidation. Tutorials with periodic concept check questions take students through the pathways step by step.
NEW Case Studies, Justine Hine, Lafayette College
Each case study introduces students to a biochemical mystery and allows them to determine what investigations will solve it. Accompanying assessments ensure that students have fully completed and understood the case study. Topics include:
• A Likely Story: Enzyme Inhibition
• An Unexplained Death: Carbohydrate Metabolism
• A Day at the Beach: Lipid Metabolism
• Runner's Experiment: Integration of Metabolism
UPDATED Animated Mechanism Figures
Every mechanism figure from the text is available as an animation, complete with explanatory narration and assessment with targeted feedback.
Animated Biochemical Techniques
Ten technique animations reveal experimental techniques available to researchers today.
UPDATED Molecular Structure Tutorials—now in JSmol
These tutorials now include assessment with targeted feedback to ensure that students get a deeper understanding of molecular structures. Tutorials include:
     • Protein Architecture (Chapter 3)
     • Oxygen-Binding Proteins (Chapter 5)
     • Major Histocompatibility Complex (MHC) Molecules (Chapter 5)
     • Nucleotides: Building Blocks of Nucleic Acids (Chapter 8)
     • Trimeric G Proteins (Chapter 12)
     • Bacteriorhodopsin (Chapter 19)
     • Restriction Endonucleases (Chapter 25)
     • The Hammerhead Ribozyme: An RNA Enzyme (Chapter 26)
     • Lac Repressor: A Gene Regulator (Chapter 28)
NEW Simulations
Based on figures in the text, these biochemical simulations let students interact with biochemical structures and processes. Simulations include:
     • Nucleotide Structure
     • DNA/RNA Structure
     • PCR
     • Sanger Sequencing
     • CRISPR
     • DNA Replication
     • DNA Polymerase
     • Mutation and Repair
     • Transcription
     • mRNA Processing
     • Translation
Living Graphs and Equations
These interactive tools, with manipulable variables, help students understand concepts and work through problems. Topics include:
     • Henderson-Hasselbalch Equation (Equation 2-9)
     • Titration Curve for a Weak Acid (Figure 2.17)
     • Binding Curve for Myoglobin (Equation 5-11)
     • Cooperative Ligand Binding (Equation 5-14)
     • Hill Equation (Equation 5-16)
     • Protein-Ligand Interactions (Equation 5.8)
     • Competitive Inhibitor (Equation 6-28)
     • Lineweaver-Burk Equation (Box 6-1)
     • Michaelis-Menten Equation (Equation 6-9)
     • Mixed Inhibitor (Equation 6-30)
     • Uncompetitive Inhibitor (Equation 6.29)
     • Free-Energy for Transport Equation (Equation 11-3)
     • Free-Energy for Transport Graph (Equation 11.3)
     • Free-Energy Change (Equation 13-4)
     • Free-Energy of Hydrolysis of ATP Equation (Worked Example 13-2)
     • Free-Energy of Hydrolysis of ATP Graph (Worked Example 13-2)
     • Free-Energy for Transport of an Ion (Equation 11.4 and Equation 19.8)
Problem-Solving Videos, Scott Ensign of Utah State University
With diagrams, graphs, and narration, these videos walk students through problems on topics that typically prove difficult, helping them understanding the right approach to the solution.
For Instructors (also available at
      • Test Bank
in editable Microsoft Word and Diploma formats includes 30 to 50 new multiple-         choice and short answer problems per chapter, for a total of more than 100 questions per-         chapter, each rated by Bloom’s level and level of difficulty.
      • Lecture Slides for the new edition, with updated, optimized art and editable text.
      • Clicker Questions for iClicker or other classroom response systems, written to foster active-              learning in the classroom and better inform instructors on student misunderstandings. 
      • Fully Optimized Art Files of every figure, photo, and table in the text feature enhanced color,-             high resolution, and enlarged fonts. These files are available as JPEGs or pre-loaded into-          Powerpoint format for each chapter.