Achieve Labs for Microbiology Simulations (1-Term Access)
First Edition| ©2022 Macmillan Learning
Assessments with error specific hints, feedback, and solutions provide students just-in-time help when they need it, regardless of when they complete their lab. The enhanced reporting features in Achieve’s gradebook help instructors identify areas where students are struggling, then provide targeted instruction/intervention, improving course retention and pass/fail rates.
Prioritizing accessibility during the product development means that accessibility isn’t just an “extra.” Macmillan Biology Lab Simulations are the most accessible in the market ensuring all students have an equal opportunity to succeed.
ISBN:9781319430313
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Achieve More with Microbiology Lab Simulations 2.0
Achieve’s open-ended microbiology lab simulations provide students with 24-hour access to realistic virtual labs. Students complete labs on their own time in a relaxed setting, and can make and learn from mistakes without worrying about wasted chemicals, time constraints, or equipment availability.
Assessments with error specific hints, feedback, and solutions provide students just-in-time help when they need it, regardless of when they complete their lab. The enhanced reporting features in Achieve’s gradebook help instructors identify areas where students are struggling, then provide targeted instruction/intervention, improving course retention and pass/fail rates.
Prioritizing accessibility during the product development means that accessibility isn’t just an “extra.” Macmillan Biology Lab Simulations are the most accessible in the market ensuring all students have an equal opportunity to succeed.
Features
Now in Achieve
Achieve is the culmination of years of development work put toward creating the most powerful online learning tool for chemistry students. It houses our renowned assessments, multimedia assets, e-lab manual, and instructor resources in a powerful new platform. Achieve supports educators and students throughout the full range of instruction including assets suitable for pre-lab preparation, in-lab e-lab manual, and post-lab assessments. The pairing of a powerful new platform with outstanding microbiology lab content provides an unrivalled learning experience.
Open Ended Lab Simulations
- The open-ended nature of these labs lets students make mistakes and learn from them just like they would in a real wet lab without the worry of wasted chemicals, lab space, or available equipment.
- Simulations are designed to improve students conceptual understanding and critical thinking skills.
- The workbench includes more than the necessary chemicals and equipment for the pre-created labs. Instructors may wish to customize the existing labs or create their own with the materials available in the virtual lab simulations.
Accessible
- Accessibility was prioritized from the start making Macmillan Lab Simulations the most accessible in the market.
- Students can access their labs at any time from various devices.
Assessments
- Assessments with error specific hints, feedback, and solutions guide students to conceptual understanding just like instructors would during office hours.
- Short answer questions give students an opportunity to demonstrate their understanding of the material.
Smart Worksheets
- Smart Worksheets provide students with error specific feedback as they complete their post-lab calculations. This immediate feedback prevents students from carrying a calculation mistake through the entire post-lab activity.
- Smart Worksheets are auto-graded, freeing up TA time to focus on student learning instead of grading.
- Smart Worksheets gradebook offers instructors a “play-back” feature that allows instructors to see a video of each student’s work during the assignment.
New to This Edition
New organization and table of contentsNew assessments for pre-lab and post-lab
Improved UX
Updated lab manual
Smart Worksheets
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Achieve Labs for Microbiology Simulations (1-Term Access)
First Edition| ©2022
Macmillan Learning
Digital Options
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Achieve
Achieve is a comprehensive set of interconnected teaching and assessment tools that incorporate the most effective elements from Macmillan Learning's market leading solutions in a single, easy-to-use platform.
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Achieve Labs for Microbiology Simulations (1-Term Access)
First Edition| 2022
Macmillan Learning
Table of Contents
Introduction
Lab Safety
Background
Procedure
Experiment 1: Safety Features and Hazards
-Part 1: Safety Features
-Part 2: Hazards
-Part 3: Leaving the Lab
Experiment 2: Dressing for the Lab
-Part 1: Dress Code
-Part 2: Personal Protective Equipment (PPE)
Introduction to Lab Simulations
Introduction to the Virtual Laboratory
Touring the Virtual Laboratory
Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH
Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas
Experiment 3: Observing a Reaction Between HCl and Zinc
Scientific Method
Background
Procedure
Experiment 1: Find the Best Mouse Diet for Weight Gain
Experiment 2: Replicate the Experiment
Basic Microscopy
Background
Procedure
Experiment 1: Visualizing Cells Using a Microscope
-Part 1: Visualizing Amoeba Cells
-Part 2: Visualizing Spirogyra Cells
-Part 3: Visualizing Cardiac Muscle Cells
-Part 4: Visualizing Bacterial Cells
Experiment 2: Estimating Size
-Part 1: Calculating Total Magnification
-Part 2: Calibration 1
-Part 3: Calibration 2
-Part 4: Estimating the Size of an Onion Epidermis Cell
-Part 5: Estimating the Width of a Blood Cell (Neutrophil)
-Part 6: Estimating the Width of a Blood Vessel
Microbiology
Aseptic Technique
Background
Procedure
Experiment 1: Pouring an Agar Plate
-Part 1: Introduction
-Part 2: Removing Agar
-Part 3: Pouring Agar
-Part 4: Summary
Experiment 2: Making a Streak Plate Using the Aseptic Technique
Cultivation of Bacteria
Background
Procedure
Experiment 1: Bacteria in the Environment
Experiment 2: Isolation of Bacteria
-Part 1: Streak Plate Method of Isolation
-Part 2: Spread Plate Method of Isolation
Staining
Background
Procedure
Experiment 1: Gram Staining
Part 1: Heat-Fixation of the S. aureus Bacterial Slide
Part 2: Gram Staining of S. aureus
Part 3: Identifying S. aureus with the Microscope
Part 4: Repeating the Gram Staining Protocol with E. coli
Experiment 2: Acid-Fast Staining
-Part 1: Heat-Fixation of the E. coli and M. phlei Bacterial Slides
-Part 2: Acid-Fast Staining of E. coli and M. phlei
Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae
Enumeration of Bacteria
Background
Procedure
Experiment 1: Spread Plating of Bacterial Serial Dilutions
Experiment 2: Counting Plate CFUs
Experiment 3: Calculating Sample CFU Density
Motility and Amino Acid Hydrolysis
Background
Procedure
Experiment 1: Motility
Experiment 2: Amino Acid Hydrolysis
Blood
Background
Procedure
Experiment 1: Normal Peripheral Blood
Experiment 2: Diseased Peripheral Blood
Unknown Identification
Background
Procedure
Experiment 1: Gram Stain
-Part 1: Heat-Fixation of the Bacterial Slide
-Part 2: Staining
-Part 3: Identifying the Bacteria with the Microscope
Experiment 2: Starch Hydrolysis Test
Experiment 3: Citrate Test
Experiment 4: Anaerobic Jar
Control of Bacterial Growth
Background
Procedure
Experiment 1: UV Radiation
Experiment 2: Chemical Agent Effects on E. coli
Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave
Bacterial Transformation
Background
Procedure
Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid
Experiment 2: Determining Transformation Success Using Selective Media
Extreme Bacteria
Background
Procedure
Experiment 1: Effect of Temperature on Microbial Growth
Experiment 2: Effect of pH on Microbial Growth
Experiment 3: Effect of Osmotic Pressure on Microbial Growth
Experiment 4: Effect of Oxygen on Microbial Growth
Antibiotic Sensitivity
Background
Procedure
Experiment 1: Performing a Disk Diffusion Test with E. coli
Experiment 2: Performing a Disk Diffusion Test with S. aureus
Experiment 3: Performing a Disk Diffusion Test with MRSA
Bacteria
Background
Procedure
Experiment 1: Performing Gram Staining of S. aureus and E. coli
-Part 1: Heat-Fixation of S. aureus
-Part 2: Gram Staining of S. aureus
-Part 3: Visualizing S. aureus with the Microscope
-Part 4: Repeating the Gram Staining Protocol with the E. coli
Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics
-Part 1: Performing a Disk Diffusion Test with Gram-Positive Bacteria
-Part 2: Repeat the Disk Diffusion Test with Gram-Negative Bacteria
Diversity
Protists
Background
Procedure
Topic 1: Investigating Excavata
-Part 1: Visualizing Euglena
-Part 2: Observing Euglena Motion
Topic 2: Investigating Rhizaria
-Part 1: Visualizing Foraminifera Shells
-Part 2: Observing Foram Motion
-Part 3: Investigating Radiolarians
Topic 3: Investigating Chromalveolata
-Part 1: Investigating Diatoms
-Part 2: Investigating Paramecium
-Part 3: Investigating Stentor
-Part 4: Investigating Dinoflagellates
Topic 4: Investigating Archaeplastida
-Part 1: Investigating Spirogyra
-Part 2: Investigating Volvox
-Part 3: Investigating Seaweed
Topic 5: Investigating Amoebas
Topic 6: Investigating Choanoflagellates
Fungi
Background
Procedure
Topic 1: Investigating Phylum Basidiomycota
-Part 1: Visualizing a Whole Agaricus Mushroom Specimen
-Part 2: Visualizing Agaricus Gills
-Part 3: Visualizing Agaricus Hyphae, Basidia, and Spores
Topic 2: Investigating Phylum Ascomycota
-Part 1: Visualizing Whole Ascomycota Mushroom Specimens
-Part 2: Visualizing Aspergillus
-Topic 3: Investigating Phylum Zygomycota
Chemistry
Acids, Bases, and pH Buffers
Background
Procedure
Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue
Experiment 2: The Phosphate Buffer System
-Part 1: Measuring pH Changes Following the Addition of HCl
-Part 2: Measuring pH Changes Following the Addition of NaOH
Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer
-Part 1: Addition of Acid
-Part 2: Addition of Base
Macromolecules
Biological Molecules
Background
Procedure
Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution
Experiment 2: Testing for Starch Using Lugol’s Iodine
Experiment 3: Testing for Lipids Using Sudan III Solution
Experiment 4: Testing for Proteins Using Biuret Solution
Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins
- Part 1: Testing Potato Juice
- Part 2: Testing Onion Juice
- Part 3: Testing Whole Milk
- Part 4: Testing Skim Milk
Enzymes
Background
Procedure
Experiment 1: Determining the Effect of Temperature on Catalase Activity
- Part 1: Determining the Effect of 10 °C on Catalase Activity
- Part 2: Determining the Effect of 21.5 °C on Catalase Activity
- Part 3: Determining the Effect of 40 °C on Catalase Activity
- Part 4: Determining the Effect of 60 °C on Catalase Activity
- Part 5: Determining the Effect of 80 °C on Catalase Activity
Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity
- Part 1: Testing the Catalase Activity of Test
-Tube 1
- Part 2: Testing the Catalase Activity of Test
-Tube 2
- Part 3: Testing the Catalase Activity of Test
-Tube 3
- Part 4: Testing the Catalase Activity of Test
-Tube 4
Experiment 3: Determining the Effect of pH on Catalase Activity
- Part 1: Testing Catalase Activity at pH 2
- Part 2: Testing Catalase Activity at pH 6
- Part 3: Testing Catalase Activity at pH 10
Quantitative Analysis of Enzyme Activity
Background
Procedure
Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements
- Part 1: Preparing a Set of Three Standards of Known Starch Concentration
- Part 2: Measuring Absorbance of the Three Standards
Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity
- Part 1: Preparing the Reaction Solutions and Measuring Their pH
- Part 2: Measuring Absorbance of Test Tube 1 After an Amylase Hydrolysis Reaction
- Part 3: Measuring Absorbance of Test Tube 2 After an Amylase Hydrolysis Reaction
- Part 4: Measuring Absorbance of Test Tube 3 After an Amylase Hydrolysis Reaction
- Part 5: Measuring Absorbance of Test Tube 4 After an Amylase Hydrolysis Reaction
- Part 6: Measuring Absorbance of Test Tube 5 After an Amylase Hydrolysis Reaction
Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity
- Part 1: Determining the Effect of 10 °C on Amylase Enzyme Activity
- Part 2: Determining the Effect of 37 °C on Amylase Enzyme Activity
- Part 3: Determining the Effect of 50 °C on Amylase Enzyme Activity
- Part 4: Determining the Effect of 80 °C onAmylase Enzyme Activity
Cells
Diffusion and Osmosis
Background
Procedure
Experiment 1: Qualitative Evidence for Diffusion
Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis
Experiment 3: Visualizing Osmosis in Living Cells
Expanded Diffusion and Osmosis
Background
Procedure
Experiment 1: Qualitative Evidence for Diffusion
Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient
Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein
- Part 1: Quantifying Weight Changes to Confirm Osmosis
- Part 2: Screening for the Presence of Protein with a Biuret Test to Confirm Osmosis
Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis
Metabolism
Cellular Respiration
Background
Procedure
Experiment 1: Fermentation of Different Sugars by Yeast Cells
- Part 1: Measuring Fermentation of Glucose
- Part 2: Measuring Fermentation of Fructose
- Part 3: Measuring Fermentation of Maltose
- Part 4: Measuring Fermentation of Maltotriose
Genetics
DNA
Background
Procedure
Experiment 1: Running a Gel Electrophoresis of DNA VNTR Fragments
Regulation of Gene Expression
Background
Procedure
Topic 1: Investigating Gene Expression
- Part 1: Transcribing Gene 1
- Part 2: Translating Gene 1
- Part 3: Regulating Gene 1
- Part 4: Transcribing Gene 2
- Part 5: Translating Gene 2
- Part 6: Regulating Gene 2
- Part 7: Transcribing Gene 3
- Part 8: Translating Gene 3
- Part 9: Regulating Gene 3
Topic 2: Investigating mRNA Production
Topic 3: Optimizing Protein Production Using Multiple Cells
Biotechnology
PCR
Background
Procedure
Experiment 1: Running a PCR
Experiment 2: Conducting Gel Electrophoresis
Nucleic Acid Assays
Background
Procedure
Experiment 1: Performing an RNA Extraction from Animal Cells
- Part 1: Resuspending the Pellet Containing RNA
- Part 2: Performing Cell Lysis
- Part 3: Homogenizing RNA
- Part 4: Purifying RNA
- Part 5: Summarizing the RNA Extraction Steps
Experiment 2: Cloning
Experiment 3: Next Generation Sequencing
- Part 1: Understanding Next Generation Sequencing
- Part 2: Preparing the Sample
- Part 3: Loading Components Into the MiSeq
- Part 4: Performing Cluster Generation
- Part 5: Sequencing by Synthesis
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Achieve Labs for Microbiology Simulations (1-Term Access)
First Edition| 2022
Macmillan Learning
Authors
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Macmillan Learning
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Achieve Labs for Microbiology Simulations (1-Term Access)
First Edition| 2022
Macmillan Learning
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