Achieve Labs for Microbiology Simulations (1-Term Access) by Macmillan Learning - First Edition, 2022 from Macmillan Student Store

Achieve Labs for Microbiology Simulations (1-Term Access)

First Edition ©2022 Macmillan Learning

Achieve’s open-ended microbiology lab simulations provide students with 24-hour access to realistic virtual labs. Students complete labs on their own time in a relaxed setting, and can make and learn from mistakes without worrying about wasted chemicals, time constraints, or equipment availability.&...
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Achieve Labs for Microbiology Simulations (1-Term Access) by Macmillan Learning - First Edition, 2022 from Macmillan Student Store

Achieve More with Microbiology Lab Simulations  2.0

Achieve’s open-ended microbiology lab simulations provide students with 24-hour access to realistic virtual labs. Students complete labs on their own time in a relaxed setting, and can make and learn from mistakes without worrying about wasted chemicals, time constraints, or equipment availability. 
 
Assessments with error specific hints, feedback, and solutions provide students just-in-time help when they need it, regardless of when they complete their lab. The enhanced reporting features in Achieve’s gradebook help instructors identify areas where students are struggling, then provide targeted instruction/intervention, improving course retention and pass/fail rates.
 
Prioritizing accessibility during the product development means that accessibility isn’t just an “extra.” Macmillan Biology Lab Simulations are the most accessible in the market ensuring all students have an equal opportunity to succeed.

Features

Now in Achieve
Achieve is the culmination of years of development work put toward creating the most powerful online learning tool for chemistry students. It houses our renowned assessments, multimedia assets, e-lab manual, and instructor resources in a powerful new platform. Achieve supports educators and students throughout the full range of instruction including assets suitable for pre-lab preparation, in-lab e-lab manual, and post-lab assessments. The pairing of a powerful new platform with outstanding microbiology lab content provides an unrivalled learning experience.

Open Ended Lab Simulations

  • The open-ended nature of these labs lets students make mistakes and learn from them just like they would in a real wet lab without the worry of wasted chemicals, lab space, or available equipment.
  • Simulations are designed to improve students' conceptual understanding and critical thinking skills.
  • The workbench includes more than the necessary chemicals and equipment for the pre-created labs. Instructors may wish to customize the existing labs or create their own with the materials available in the virtual lab simulations.

Accessible

  • Accessibility was prioritized from the start making Macmillan Lab Simulations the most accessible in the market.
  • Students can access their labs at any time from various devices.

Assessments

  • Assessments with error specific hints, feedback, and solutions guide students to conceptual understanding just like instructors would during office hours.
  • Short answer questions give students an opportunity to demonstrate their understanding of the material.

Smart Worksheets

  • Smart Worksheets provide students with error specific feedback as they complete their post-lab calculations. This immediate feedback prevents students from carrying a calculation mistake through the entire post-lab activity.
  • Smart Worksheets are auto-graded, freeing up TA time to focus on student learning instead of grading.
  • Smart Worksheets gradebook offers instructors a “play-back” feature that allows instructors to see a video of each student’s work during the assignment.

New to This Edition

New organization and table of contents
New assessments for pre-lab and post-lab
Improved UX
Updated lab manual
Smart Worksheets

Digital Options

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Achieve

Achieve is a comprehensive set of interconnected teaching and assessment tools that incorporate the most effective elements from Macmillan Learning's market leading solutions in a single, easy-to-use platform.

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Table of Contents

Introduction

Lab Safety

Background

Procedure

Experiment 1: Safety Features and Hazards

-Part 1: Safety Features

-Part 2: Hazards

-Part 3: Leaving the Lab

Experiment 2: Dressing for the Lab

-Part 1: Dress Code

-Part 2: Personal Protective Equipment (PPE)

Introduction to Lab Simulations

Introduction to the Virtual Laboratory

Touring the Virtual Laboratory

Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH

Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas

Experiment 3: Observing a Reaction Between HCl and Zinc

Scientific Method

Background

Procedure

Experiment 1: Find the Best Mouse Diet for Weight Gain

Experiment 2: Replicate the Experiment

Basic Microscopy

Background

Procedure

Experiment 1: Visualizing Cells Using a Microscope

-Part 1: Visualizing Amoeba Cells

-Part 2: Visualizing Spirogyra Cells

-Part 3: Visualizing Cardiac Muscle Cells

-Part 4: Visualizing Bacterial Cells

Experiment 2: Estimating Size

-Part 1: Calculating Total Magnification

-Part 2: Calibration 1

-Part 3: Calibration 2

-Part 4: Estimating the Size of an Onion Epidermis Cell

-Part 5: Estimating the Width of a Blood Cell (Neutrophil)

-Part 6: Estimating the Width of a Blood Vessel

Microbiology

Aseptic Technique

Background

Procedure

Experiment 1: Pouring an Agar Plate

-Part 1: Introduction

-Part 2: Removing Agar

-Part 3: Pouring Agar

-Part 4: Summary

Experiment 2: Making a Streak Plate Using the Aseptic Technique

Cultivation of Bacteria

Background

Procedure

Experiment 1: Bacteria in the Environment

Experiment 2: Isolation of Bacteria

-Part 1: Streak Plate Method of Isolation

-Part 2: Spread Plate Method of Isolation

Staining

Background

Procedure

Experiment 1: Gram Staining

Part 1: Heat-Fixation of the S. aureus Bacterial Slide

Part 2: Gram Staining of S. aureus

Part 3: Identifying S. aureus with the Microscope

Part 4: Repeating the Gram Staining Protocol with E. coli

Experiment 2: Acid-Fast Staining

-Part 1: Heat-Fixation of the E. coli and  M. phlei Bacterial Slides

-Part 2: Acid-Fast Staining of E. coli and M. phlei

Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae

Enumeration of Bacteria

Background

Procedure

Experiment 1: Spread Plating of Bacterial Serial Dilutions

Experiment 2: Counting Plate CFUs

Experiment 3: Calculating Sample CFU Density

Motility and Amino Acid Hydrolysis

Background

Procedure

Experiment 1: Motility

Experiment 2: Amino Acid Hydrolysis

Blood

Background

Procedure

Experiment 1: Normal Peripheral Blood

Experiment 2: Diseased Peripheral Blood

Unknown Identification

Background

Procedure

Experiment 1: Gram Stain

-Part 1: Heat-Fixation of the Bacterial Slide

-Part 2: Staining

-Part 3: Identifying the Bacteria with the Microscope

Experiment 2: Starch Hydrolysis Test

Experiment 3: Citrate Test

Experiment 4: Anaerobic Jar

Control of Bacterial Growth

Background

Procedure

Experiment 1: UV Radiation

Experiment 2: Chemical Agent Effects on E. coli

Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave

Bacterial Transformation

Background

Procedure

Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid

Experiment 2: Determining Transformation Success Using Selective Media

Extreme Bacteria

Background

Procedure

Experiment 1: Effect of Temperature on Microbial Growth

Experiment 2: Effect of pH on Microbial Growth

Experiment 3: Effect of Osmotic Pressure on Microbial Growth

Experiment 4: Effect of Oxygen on Microbial Growth

Antibiotic Sensitivity

Background

Procedure

Experiment 1: Performing a Disk Diffusion Test with E. coli

Experiment 2: Performing a Disk Diffusion Test with S. aureus

Experiment 3: Performing a Disk Diffusion Test with MRSA

Bacteria

Background

Procedure

Experiment 1: Performing Gram Staining of S. aureus and E. coli

-Part 1: Heat-Fixation of S. aureus

-Part 2: Gram Staining of S. aureus

-Part 3: Visualizing S. aureus with the Microscope

-Part 4: Repeating the Gram Staining Protocol with the E. coli

Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics

-Part 1: Performing a Disk Diffusion Test with Gram-Positive Bacteria

-Part 2: Repeat the Disk Diffusion Test with Gram-Negative Bacteria

Diversity

Protists

Background

Procedure

Topic 1: Investigating Excavata

-Part 1: Visualizing Euglena

-Part 2: Observing Euglena Motion

Topic 2: Investigating Rhizaria

-Part 1: Visualizing Foraminifera Shells

-Part 2: Observing Foram Motion

-Part 3: Investigating Radiolarians

Topic 3: Investigating Chromalveolata

-Part 1: Investigating Diatoms

-Part 2: Investigating Paramecium

-Part 3: Investigating Stentor

-Part 4: Investigating Dinoflagellates

Topic 4: Investigating Archaeplastida

-Part 1: Investigating Spirogyra

-Part 2: Investigating Volvox

-Part 3: Investigating Seaweed

Topic 5: Investigating Amoebas

Topic 6: Investigating Choanoflagellates

Fungi

Background

Procedure

Topic 1: Investigating Phylum Basidiomycota

-Part 1: Visualizing a Whole Agaricus Mushroom Specimen

-Part 2: Visualizing Agaricus Gills

-Part 3: Visualizing Agaricus Hyphae, Basidia, and Spores

Topic 2: Investigating Phylum Ascomycota

-Part 1: Visualizing Whole Ascomycota Mushroom Specimens

-Part 2: Visualizing Aspergillus

-Topic 3: Investigating Phylum Zygomycota

Chemistry

Acids, Bases, and pH Buffers

Background

Procedure

Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue

Experiment 2: The Phosphate Buffer System

-Part 1: Measuring pH Changes Following the Addition of HCl

-Part 2: Measuring pH Changes Following the Addition of NaOH

Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer

-Part 1: Addition of Acid

-Part 2: Addition of Base

Macromolecules

Biological Molecules

Background

Procedure

Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution

Experiment 2: Testing for Starch Using Lugol’s Iodine

Experiment 3: Testing for Lipids Using Sudan III Solution

Experiment 4: Testing for Proteins Using Biuret Solution

Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins

  • Part 1: Testing Potato Juice
  • Part 2: Testing Onion Juice
  • Part 3: Testing Whole Milk
  • Part 4: Testing Skim Milk

Enzymes

Background

Procedure

Experiment 1: Determining the Effect of Temperature on Catalase Activity

  • Part 1: Determining the Effect of 10 °C on Catalase Activity
  • Part 2: Determining the Effect of 21.5 °C on Catalase Activity
  • Part 3: Determining the Effect of 40 °C on Catalase Activity
  • Part 4: Determining the Effect of 60 °C on Catalase Activity
  • Part 5: Determining the Effect of 80 °C on Catalase Activity

Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity

  • Part 1: Testing the Catalase Activity of Test

 -Tube 1

  • Part 2: Testing the Catalase Activity of Test

-Tube 2

  • Part 3: Testing the Catalase Activity of Test

-Tube 3

  • Part 4: Testing the Catalase Activity of Test

-Tube 4

Experiment 3: Determining the Effect of pH on Catalase Activity

  • Part 1: Testing Catalase Activity at pH 2
  • Part 2: Testing Catalase Activity at pH 6
  • Part 3: Testing Catalase Activity at pH 10

Quantitative Analysis of Enzyme Activity

Background

Procedure

Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements

  • Part 1: Preparing a Set of Three Standards of Known Starch Concentration
  • Part 2: Measuring Absorbance of the Three Standards

Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity

  • Part 1: Preparing the Reaction Solutions and Measuring Their pH
  • Part 2: Measuring Absorbance of Test Tube 1 After an Amylase Hydrolysis Reaction
  • Part 3: Measuring Absorbance of Test Tube 2 After an Amylase Hydrolysis Reaction
  • Part 4: Measuring Absorbance of Test Tube 3 After an Amylase Hydrolysis Reaction
  • Part 5: Measuring Absorbance of Test Tube 4 After an Amylase Hydrolysis Reaction
  • Part 6: Measuring Absorbance of Test Tube 5 After an Amylase Hydrolysis Reaction

Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity

  • Part 1: Determining the Effect of 10 °C on Amylase Enzyme Activity
  • Part 2: Determining the Effect of 37 °C on Amylase Enzyme Activity
  • Part 3: Determining the Effect of 50 °C on Amylase Enzyme Activity
  • Part 4: Determining the Effect of 80 °C onAmylase Enzyme Activity

Cells

Diffusion and Osmosis

Background

Procedure

Experiment 1: Qualitative Evidence for Diffusion

Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis

Experiment 3: Visualizing Osmosis in Living Cells

Expanded Diffusion and Osmosis

Background

Procedure

Experiment 1: Qualitative Evidence for Diffusion

Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient

Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein

  • Part 1: Quantifying Weight Changes to Confirm Osmosis
  • Part 2: Screening for the Presence of Protein with a Biuret Test to Confirm Osmosis

Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis

Metabolism

Cellular Respiration

Background

Procedure

Experiment 1: Fermentation of Different Sugars by Yeast Cells

  • Part 1: Measuring Fermentation of Glucose
  • Part 2: Measuring Fermentation of Fructose
  • Part 3: Measuring Fermentation of Maltose
  • Part 4: Measuring Fermentation of Maltotriose

Genetics

DNA

Background

Procedure

Experiment 1: Running a Gel Electrophoresis of DNA VNTR Fragments

Regulation of Gene Expression

Background

Procedure

Topic 1: Investigating Gene Expression

  • Part 1: Transcribing Gene 1
  • Part 2: Translating Gene 1
  • Part 3: Regulating Gene 1
  • Part 4: Transcribing Gene 2
  • Part 5: Translating Gene 2
  • Part 6: Regulating Gene 2
  • Part 7: Transcribing Gene 3
  • Part 8: Translating Gene 3
  • Part 9: Regulating Gene 3

Topic 2: Investigating mRNA Production

Topic 3: Optimizing Protein Production Using Multiple Cells

Biotechnology

PCR

Background

Procedure

Experiment 1: Running a PCR

Experiment 2: Conducting Gel Electrophoresis

Nucleic Acid Assays

Background

Procedure

Experiment 1: Performing an RNA Extraction from Animal Cells

  • Part 1: Resuspending the Pellet Containing RNA
  • Part 2: Performing Cell Lysis
  • Part 3: Homogenizing RNA
  • Part 4: Purifying RNA
  • Part 5: Summarizing the RNA Extraction Steps

Experiment 2: Cloning

Experiment 3: Next Generation Sequencing

  • Part 1: Understanding Next Generation Sequencing
  • Part 2: Preparing the Sample
  • Part 3: Loading Components Into the MiSeq
  • Part 4: Performing Cluster Generation
  • Part 5: Sequencing by Synthesis

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